The Preparation and Biochemical Properties of Mitochondria from Neurospora crassa
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چکیده
منابع مشابه
Formation of Mitochondria in Neurospora Crassa
The choline concentration used in the growth medium influences the density of mitochondria produced by the chol-1 mutant of Neurospora. Isopycnic centrifugation in sucrose gradients can be used to determine the density of mitochondria, and can resolve into two populations, mitochondria derived from a mixture of cells grown at low (1 microg/ml choline chloride) and high (10 microg/ml choline chl...
متن کاملSuperoxide dismutases in mitochondria from Helianthls tuberosus and Neurospora crassa.
Superoxide dismutase has been studied in a wide range of organisms (Fridovich, 1975). Early observations suggested that superoxide dismutases isolated from eukaryotes (McCord & Fridovich, 1969; Misra & Fridovich, 1972; Beauchamp & Fridovich, 1973) showed little diversity in their properties, having mol.wts. near 32000 and containing two copper and two zinc atoms per molecule. The activity of th...
متن کاملFormation of Mitochondria in Neurospora Crassa
Cells of a choline-requiring mutant of Neurospora crassa, labeled with radioactive choline, were transferred to unlabeled medium. At various times during their subsequent logarithmic growth, a highly purified mitochondrial fraction was prepared by sucrose density gradient centrifugation, and the distribution of label among individual mitochondria was determined by quantitative autoradiography. ...
متن کاملThe purification and properties of superoxide dismutase from Neurospora crassa.
Soluble extracts of Neurospora crassa contain a single, electrophoretically distinct, superoxide dismutase. This enzyme has been isolated and has been found to be a bluegreen, copperand zinc-containing enzyme, similar to that already described from bovine tissues and from garden peas. The molecular weight was ,approximately 31,000, and the enzyme appeared to be composed of 2 subunits of equal s...
متن کاملThe purification and properties of histidinol dehydrogenase from Neurospora crassa.
1. A procedure is described for the purification of l-histidinol dehydrogenase (l-histidinol-NAD oxidoreductase, EC 1.1.1.23) from Neurospora crassa. 2. The enzyme, as purified, has a sedimentation coefficient, S(20), of 7.1s and a molecular weight of 81 000. Considerable variation is possible in the state of polymerization of the enzyme, giving rise to observed molecular weights from 40 000 to...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1967
ISSN: 0022-1287
DOI: 10.1099/00221287-48-3-419